Measurement of anti proliferative activity experiment

All the experiments were repeated three times. The image was recorded by Spot 32 image capture software Diagnostic Instruments.

The studies suggested that sinigrin could inhibit the cancer cell growth. Animal Treatment Sprague Dawley SD rats were induced with carcinogens in the in vivo experiments according to the previously established method [11].

Finally, the slides were mounted with cover-slip and allowed to dry. Received Dec 15; Accepted Mar Copyright Wang et al. Absorbance was measured at nm. These data suggest that liver functions can be gradually restored after treatment with sinigrin.

Comparison of assays was made by correlation and linear regression analysis. Animal Treatment Sprague Dawley SD rats were induced with carcinogens in the in vivo experiments according to the previously established method [11]. The body weight of the positive control group was significantly reduced.

The protocol of the experiment was the same as for the AST assay. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Chinese University of Hong Kong.

Anti-Proliferative Activities of Sinigrin on Carcinogen-Induced Hepatotoxicity in Rats

These studies reflect the health benefits of sinigrin towards carcinogens-induced liver injury. Sinigrin is one of the major ingredients present in Brassica nigra, which has been used in combination with other herbs for treatment of various diseases.

Slides were washed with three changes of PBS for 5 minutes each. Effects of sinigrin treatment on the liver weight of different groups of rats are shown in Figure 3.

IC50 values were calculated by Probit regression. Fifty rats were randomly divided into 3 groups, negative control normal group 10 ratsa positive control untreated group 10 rats and three sinigrin-treatment groups 10 rats each.

Glucosinolates have been reported to exhibit different pharmacological properties in vitro [1] — [4]. The washing solution was centrifuged at 1, rpm for 3 minutes. Vacutainers were then centrifuged at 3, rpm for 15 minutes.

Piwen WangSusanne M. The results suggest that sinigrin exerts important anti-proliferative activities in carcinogen-induced hepatocarcinogenesis in rats, and highlight the potential of sinigrin as an anti-cancer agent for liver cancer.

Tens of thousands of cells were seeded in well plates. In order to compare the efficacy of sinigrin with a common cancer drug, doxorubicin was used to treat the carcinogens-induced hepatocarcinogenesis in the rat. Fifty microliters of Neutral Red solution was added to each well.

Statistics One-way ANOVA was performed to determine the statistical significance of differences among treated and untreated groups.

The results suggest that sinigrin exerts important anti-proliferative activities in carcinogen-induced hepatocarcinogenesis in rats, and highlight the potential of sinigrin as an anti-cancer agent for liver cancer.

The liver weight index of doxorubicin-treated group was similar to that of the positive control group. The image of the slides were checked and captured by an Axiophot-2 Universal microscope Zeiss connected with a computer for documentation.

Anti-Proliferative Activities of Sinigrin on Carcinogen-Induced Hepatotoxicity in Rats

These studies reflect the health benefits of sinigrin towards carcinogens-induced liver injury. The image with 2.Anti-proliferative activity-guided isolation experiments led to the purification of a clerodane diterpenoid CDA from Salvia nemorosa.

MTT, DNA ladder, DAPI staining, cell cycle analysis, and annexin V/PI assays indicated that clerodermic acid has strong geno- and cytotoxicity and induces apoptosis in A cells. The results from the anti-proliferative activity assays are shown in Table 1, Table 2.

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All the compounds can be divided into three groups, namely 4, 5 and 6, according to their structural scaffolds. When tested against the SK-N-MC and HCT cell lines, compounds 4a and 4b had limited anti-proliferative activity. The anti-proliferative activities of sinigrin were studied in a model of carcinogen-induced hepatotoxicity in rats.

Rats were orally administered with sinigrin on a daily basis for three months before sacrifice. A colorimetric sulforhodamine B (SRB) assay was used for the measurement of anti-proliferative activity as described before (Adaramoye et al., ; Fricker and Buckley, ; Keepers et al., ; Skehan et al., ).

It is the second major technique for testing and is the more preferred. Various natural compounds were reported to persuade cell cytotoxicity through targeting and downregulation of the HIF The genus Salvia is a rich source of bioactive terpenoids which show promising anti-cancer activities.

Here, the identification of natural anti-proliferative compound targeting the HIF-1α expression was reported. Cell cycle, Annexin-V/7-aminoactinomycin D staining, and measurement of activation and inhibition of caspase enzymes were also done to validate the anti-proliferative activity. The results of the experiment show that M.

ptelefolia significant antioxidant, anti-proliferative, apoptosis induction, and cancer cell cycle inhibition activities.

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Measurement of anti proliferative activity experiment
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